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Microphoto attachment МФН-12 (MFN-12)
Technical description and operation instructions

Due to continuous improvement of the attachment, minor modifications in its design may be omitted in the Technical Description as not affecting quality of operation and operation rules.
TechDesc & instruction MFN-12

1. DESIGNATION

The microphoto attachment МФН-12 is denoted for photographing of objects which are examined on the biological microscope as in the transmitted as well as in the reflected light.

A film camera (without an objective) is used with the microphoto attachment.

Microphoto attachment is a universal one as per its design shape and it fits all stands of microscopes having the proper recess for the eyepiece tubuses.

The microphoto attachment is produced for work in macroclimate regions with temperate climate in laboratory premises at air temperature from +10 to +35° C.

2. MAIN DATA

Limits of the dioptrical focusing of the eyepiece, dptr±5
Huygens eyepiece 7x
(focal distance / visual field), mm
36 / 18
Compensation eyepiece 10x
(focal distance / visual field), mm
25,2 / 13
Size of frame, mm24x36
Overall dimensions, mm140x145x260
Massa, kg1,4

3. DESIGN AND OPERATION

3.1. Optical Schema

Fig.1. Optical  Schema

Rays of light coming from photoeyepiece 1 (fig. 1) are divided into two pencils with prism 2. The major part of the light flow (about 60%) passes through lens 2 onto photofilm 3. The rest part is reflected from the prism at 20° and directed in the eyepiece 4 of the visual tube.

Lens 5 of the visual tube brings the reflexion of the object onto the plane of grid 6 placed in the focal plane of eyepiece 4. Grid 6 is installed in such a way that the reflexion of the object image is got sharp as on the photofilm as well as on the grid.

Eyepiece 4 of the visual tube can be shifted within ±5D for focusing onto the grid in accordance with eyes. The rectangular (film gate) and four double lines are put down on the reticle. They serve for more accurate focusing of the eyepiece.

Optical scheme of the attachment is made so, that the part of the reflexion of the object image limited with the film gate in sight of the visual tube is fully placed on the film in the frame of 24mm x 36mm.

3.2. Design

General view of the microphoto attachment is given in fig. 2.

Fig.2. Design (External view) МФН-12

General view of the microphoto attachment is given in fig. 2.

Vertical tubus 7 is installed in the recess under the eyepiece tubus so that the eyepiece bush is to be set from upwards.

The low setting flange of attachment's body 8 is placed on the upper conical boring of tubus 7 and is fixed with screw 9.

Light-dividing prism 2 (fig. 1) is fixed in the body of the attachment.

Inside visual tube 10 (fig. 2) lens 5 (fig. 1), grid 6 and eyepieces 4 are placed.

When dioptrical bush 11 (fig. 2) is turned taking hold on its knurling the eyepiece is shifted along the thread with respect to the optical axis Tor adjustment of it on sharpness of the grid.

Smoke-coloured light filter 12 is put on the eyepiece for protection of eyes from the bright light when looking into the visual tube.

Screw 13 serves for adjusting of the grid at the plant-manufacturer, that's why it can be unscrewed in no case due to the shifting of the grid. The grid is put in such a way that the sharp reflexion of the object image is got simultaneously as on the grid as well as on the film.

Camera 15 with cable release 16 screwed into it is fastened to barrel 14 on the attachment housing. In contrast with the standard cable release, cable release 16 has a device which helps to make the shutter, set to the exposure «B», be automatically open during a long period of time. If washer 17, the tail of which is inserted into the cable release head, is pressed against the cable release head and slightly turned clockwise, the cable release will operate as standard one. If the washer is turned counterclockwise, the knob will not return to the initial position after being pressed and the shutter exposure «B» becomes a long exposure. Only after additional pressing washer 17 the cable release knob returns to the initial position and closes the shutter.

4. MARKING

Every attachment bears the following designations: Manufacturer's trade mark, symbol and item number. The first two digits of the item number stand for the two last digits of the year of output.

5. OPERATION PROCEDURE

Correct adjustment of illumination and proper opening of field and aperture diaphragms are of considerable importance for microscope resolution and quality of photos, so it is necessary to adjust the microscope carefully before photographing.

5.1. Simultaneous Observation and Photographing

During assembling of the attachment and installation of it on the microscope, the inclined tubus is taken from the microscope and the vertical tubus 7 is put on its place. An eyepiece from the set is inserted in the upper plug of the tubus 7. Body 8 of the attachment is to be installed on the upper conical boring of the tubus and then to be tightened with the screw 9.

For work with the microphoto attachment first of all the microscope must be adjusted according to the description of the microscope; by way of rotating of dioptrical plug 11 the eyepiece of the visual tube of the attachment is set for sharp reflexion of the grid in accordance with the eye. In this case the reflexion of the grid is considered to be sharp if an observer sees the reticules of the grid separately and clearly.

Then, watching in the visual tubus of the attachment, the microscope is focused onto the object by means of coarse and micrometric focusing.

Sharpness of reflexion image of the field stop is to be checked up. The best lightening of the object and the resolution of its structure are to be obtained. A light filter must be chosen and installed under the condenser of the microscope. As per the scale of exposure time of camera 15 the required rate of blow is to be installed, cable 16 is to be screwed in the camera, the shutter is to be cocked and photographing is to be started, following the description of the photographic camera.

5.2. Determination of Magnification

The magnification (Г) on the camera film is calculated from the following formula:

Г = Vob • Гoc • 0,43,

where Vob — linear objective magnification; Гoc — visual eyepiece magnification; 0,43 — adjustment coefficient.

Visual tube of attachment has proper magnification 2,5, hetherto visual observation magnification will be:

Гattach. = Vob • Гob • 0,43 • 2,5.

Example of calculation of magnification (objective 40X, eyepiece 10x):
Magnification on camera film Г = 40•10•0,43 = 172,
Magnification at visual observation Гattach. = 40•10•0,43•2,5 = 430.

Object-micrometer is used for accurate determination of magnification.

6. CARE OF MICROPHOTO ATTACHMENT, STORAGE AND TRANSPORTATION

6.1. Care

The attcahment requires careful handling.

The attachment should be wiped periodically with a soft rag soaked in acidless vaseline, then with dry soft clean rag.

Pay special attention to cleanness of optical parts.

Never touch the lens surfaces with fingers.

Dust the outer surfaces of lenses with a very soft brush washed in ether. For subsequent cleaning, if required, wipe them slightly with a soft, many times washed linen or cambric rag slightly moistened with benzine, anaesthetic ether or xylene.

6.2. Storage

Keep the attachment in dry, clean and warm premises.

Cover it, when inoperative, with a napkin or put it in its casing.

6.3. Transportation

For moving to another premises, put the attachment set into its wooden casing.

Take care that the attachment and its accessories never displace at shakes.

Any closed transport allowed.

7. CATALOGUE OF SPARE PARTS FOR EXTRA ORDER

Huygenian eyepiece 7xЮ-41.31.106 Сп
Compensation eyepiece 10хЮ-41.31.504 Сп
Light filter СС1Ю-24.91.101
Light filter ЗС11Ю-24.91.301
Light filter ЖС12Ю-24.91.601
Light filter ОС12Ю-24.91.801
Light filter НСЗЮ-24.92.201
Light filter НС8Ю-71.92.387
Light filter НС10Ю-71.92.388
Light filter in mountЮ-41.59.211
Vertical tubusЮ-28.54.104 Сп
 
© Leningrad' optical-mechanichal cooperation (LOMO)

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Днепропетровская государственная медицинская академия, кафедра гистологии.
Адрес: 49005, Днепропетровск, ул. Севастопольская, 17 (морфологический корпус ДГМА).
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Key words: laboratory equipment, microscopy histology, biology.   Ключевые слова: лаборатория, методики, техника, реактивы, описание приборов, инструкции, паспорт, медицина, биология, гистологические исследования, микроскопы.
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